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Quiz

Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule

Komal Kohli March 16, 2023

These are the options for the question :Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule

5'C-T-G-C-A G 3'

3 G A-C-G-T-c5

Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.

(a) 5' C-T-G     C-A-GG 3'      C5 G-C-A-G     3' 3'G A-C-G-T

b) 5 C-T            T-C5 3'             G-A-G-C

(c) C-T-G-C 3'            G-A -C-G 5'              C-T-G-C-A A-G 3

d) 3'G                   A-C-G-T-C 5

The correct answer is -(a) 5' C-T-G     C-A-GG 3'      C5 G-C-A-G     3' 3'G A-C-G-T